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Aggrecan expression

WebIn addition, we have studied the expression level of aggrecan, a proteoglycan that forms an essential part of cartilage ECM, giving cartilage tissue the ability to withstand compressive loads [49 ... WebThe localizations of aggrecan and Col-II, as well as the SUMOylation modification of these proteins were analyzed using immunofluorescence and immunoprecipitation assays, …

PI3K/AKT regulates aggrecan gene expression by modulating Sox9 ... - PubMed

WebMar 2, 2024 · (D) Immunostaining analysis showing the expression of fatty acid binding protein 4 (FABP4), osteocalcin and aggrecan in adipogenic, osteogenic, and chondrogenic induced cells, respectively. No staining has been seen for FABP4 in undifferentiated (UD) controls, but weak staining observed for osteocalcin and aggrecan in UD controls. WebApr 7, 2024 · Optimized conditions of coupled electrical field (60 kHz, 2 mV/mm cyclic stimulation) when applied for 1 h increased aggrecan mRNA expression, and for 6 h—collagen type II; 30 min continuous stimulation reduced expression of MMP-1, MMP-3, MMP-13 and ADAMTS-4, and ADAMTS-5. These effects can be completely blocked by … marinerito rafael alberti https://adellepioli.com

The development and activity-dependent expression of aggrecan …

WebDec 7, 2024 · Through gene expression profiling, it is possible to measure and compare the expression level of mRNAs in differentiation-induced cells and noninduced cells. This allows the identification of genes that are overexpressed or underexpressed in differentiation-induced cells. WebJun 30, 2024 · To determine whether matrix synthesis and degradation was affected by SNP and/or S-Equol, primary chondrocytes were treated with 0.8 mM SNP and/or 30 μM S-Equol for 24 h, then polysaccharides contents were examined by toluidine blue O staining, type II collagen, and aggrecan gene expression were analyzed by real-time PCR, and MMP-2, … Aggrecan is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing. Aggrecan was named for its ability to form large aggregates in the cartilage tissue (a large aggregating … See more Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the See more Aggrecan is a high molecular weight (1x10 < M < 3x10 ) proteoglycan. It exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate glycosaminoglycan … See more The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. The linker domain between the N-terminal globular domains, called the interglobular domain, is highly … See more • Aggrecan at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • Human ACAN genome location and See more Aggrecan is a critical component for cartilage structure and the function of joints. Functionally, the G1 domain interacts with hyaluronic acid and link protein, forming stable ternary complexes in the extracellular matrix. … See more • Watanabe H, Yamada Y, Kimata K (1998). "Roles of aggrecan, a large chondroitin sulfate proteoglycan, in cartilage structure and function" See more dalton ma police chief

ACAN aggrecan [Homo sapiens (human)] - Gene - NCBI

Category:Studies on type II collagen and aggrecan production in human

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Aggrecan expression

Aggrecan - an overview ScienceDirect Topics

WebExpression of type II collagen or aggrecan was not detected immunohistochemically in skin or bone. These results demonstrate that human articular chondrocytes can be characterized in culture, by the combined application of PCR and immunohistochemistry. WebNov 15, 2024 · Previous research revealed that an imbalance in the extracellular matrix (ECM) via abnormal hypoxia-inducible factor-1alpha (HIF-1α) expression in nucleus pulposus (NP) cells was one of the key factors in the pathogenesis of intervertebral disc degeneration (IDD).

Aggrecan expression

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WebApr 10, 2024 · Gene expressions of col2a1, aggrecan and sox9 were significantly down-regulated, while expression of MMP13 was significantly up-regulated in defect group compared to control group (Fig. 1 E). Download : Download high-res image (934KB) Download : Download full-size image; Figure 1. Evaluation of perifocal chondrocyte … WebMay 4, 2024 · Aggrecan expression is seen from embryonic stages and increases with age; it is widespread, even in the normal adult retina, particularly in the synaptic layers …

WebNational Center for Biotechnology Information WebMar 11, 2024 · We investigated the association between the efficacy of NTP on aggrecan expression and the NAT2 genotype in cell donors. Methods. NP cells were isolated from intervertebral disc tissues donated by 31 Japanese patients (28–68 years) who underwent discectomy. NTP was added to the primary cell cultures and its effect on the aggrecan …

WebAggrecan is a type of protein known as a proteoglycan, which means it has several sugar molecules attached to it. It is the most abundant proteoglycan in cartilage, a tough, … WebJun 17, 2024 · Aggrecan is an integral component of the extracellular matrix in cartilaginous tissues, including the growth plate. Heterozygous defects in the aggrecan gene have …

WebFeb 28, 2024 · This suggests that eNOS deficiency may elevate aggrecan expression, which promotes apoptosis in VSMC, thereby contributing to atherosclerosis progression. These results may facilitate the development of novel approaches for improving the diagnosis or treatment of atherosclerosis.

WebExpression of constitutively active (CA)-PI3K significantly induced aggrecan promoter activity. We observed that PI3K maintained Sox9 gene expression and activity: inhibition of PI3K/AKT resulted in decreased Sox9 expression, lowered promoter activity, and mediated a reduction in Sox9 transcriptional activity. dalton ma police phone numberWebIn addition, TGF‑β1‑induced type II collagen and aggrecan expression were significantly suppressed by ERK1/2 inhibitor PD98059. Similarly, TGF‑β1‑stimulated type II collagen and aggrecan expression were decreased in the presence of a Smad3 phosphorylation inhibitor SIS3. Therefore, the present study demonstrated that the ERK1/2 and ... marine rise gullaneWebApr 14, 2000 · Aggrecan is one of the major structural components in cartilage; however, the molecular mechanism of aggrecan gene regulation has not yet been fully elucidated. TC6 is a clonal chondrocytic cell line derived from articular cartilage. mariner mall superior wisconsinWebThe localizations of aggrecan and Col-II, as well as the SUMOylation modification of these proteins were analyzed using immunofluorescence and immunoprecipitation assays, respectively.Results: Our results showed that a proinflammatory cytokine interleukin-1β induced the OA model and desumoylation of aggrecan and collagen type II because the ... mariner mall pensacola flWebSep 29, 2016 · RUNX-2 is a major transcription factor regulating aggrecan loss via the regulation of ADAMTS-4/5 gene expression [29,30] while SOX-9 acts as a master … mariner mall clinicWebOrthologous to human ACAN (aggrecan). [provided by Alliance of Genome Resources, Apr 2024] Expression Biased expression in limb E14.5 (RPKM 40.6) and CNS E14 (RPKM 4.1) See more Orthologs human all NEW Try the new Gene table Try the new Transcript table Genomic context See Acan in Genome Data Viewer Location: 7 D2; 7 44.88 cM Exon … mariner laporte inWebApr 10, 2024 · The expressions of DYRK1A and its downstream signal epidermal growth factor receptor (EGFR) were detected in the cartilage of adult wild-type mice with … marine river