WebIn addition, we have studied the expression level of aggrecan, a proteoglycan that forms an essential part of cartilage ECM, giving cartilage tissue the ability to withstand compressive loads [49 ... WebThe localizations of aggrecan and Col-II, as well as the SUMOylation modification of these proteins were analyzed using immunofluorescence and immunoprecipitation assays, …
PI3K/AKT regulates aggrecan gene expression by modulating Sox9 ... - PubMed
WebMar 2, 2024 · (D) Immunostaining analysis showing the expression of fatty acid binding protein 4 (FABP4), osteocalcin and aggrecan in adipogenic, osteogenic, and chondrogenic induced cells, respectively. No staining has been seen for FABP4 in undifferentiated (UD) controls, but weak staining observed for osteocalcin and aggrecan in UD controls. WebApr 7, 2024 · Optimized conditions of coupled electrical field (60 kHz, 2 mV/mm cyclic stimulation) when applied for 1 h increased aggrecan mRNA expression, and for 6 h—collagen type II; 30 min continuous stimulation reduced expression of MMP-1, MMP-3, MMP-13 and ADAMTS-4, and ADAMTS-5. These effects can be completely blocked by … marinerito rafael alberti
The development and activity-dependent expression of aggrecan …
WebDec 7, 2024 · Through gene expression profiling, it is possible to measure and compare the expression level of mRNAs in differentiation-induced cells and noninduced cells. This allows the identification of genes that are overexpressed or underexpressed in differentiation-induced cells. WebJun 30, 2024 · To determine whether matrix synthesis and degradation was affected by SNP and/or S-Equol, primary chondrocytes were treated with 0.8 mM SNP and/or 30 μM S-Equol for 24 h, then polysaccharides contents were examined by toluidine blue O staining, type II collagen, and aggrecan gene expression were analyzed by real-time PCR, and MMP-2, … Aggrecan is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing. Aggrecan was named for its ability to form large aggregates in the cartilage tissue (a large aggregating … See more Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the See more Aggrecan is a high molecular weight (1x10 < M < 3x10 ) proteoglycan. It exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate glycosaminoglycan … See more The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. The linker domain between the N-terminal globular domains, called the interglobular domain, is highly … See more • Aggrecan at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • Human ACAN genome location and See more Aggrecan is a critical component for cartilage structure and the function of joints. Functionally, the G1 domain interacts with hyaluronic acid and link protein, forming stable ternary complexes in the extracellular matrix. … See more • Watanabe H, Yamada Y, Kimata K (1998). "Roles of aggrecan, a large chondroitin sulfate proteoglycan, in cartilage structure and function" See more dalton ma police chief