Cisplatin a549

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August undefined, 2024

WebFeb 28, 2024 · All the new complexes showed cytotoxicity in vitro against the three cancer cell lines, but only complex 1a showed statistically significant different activity compared with cisplatin, with significantly lower IC 50 values, 4.31, 6.23, and 4.80 μM, compared with 9.71, 14.48, and 11.59 μM for cisplatin against A549, SKOV3, and MCF-7 ... WebThe A549 cells transfected with sh-PVT1 or sh-NC plasmid were seeded into the right flank of the mice, and 50 μM cisplatin was injected into the intraperitoneal every three days. …

Molecules Free Full-Text Phenethyl Isothiocyanate and Cisplatin …

WebWe used the lung cancer cell line, A549, to determine the effects of the curcuminoids and cisplatin on cell viability and several apoptotic signaling mechanisms. Cellular viability … WebFeb 22, 2024 · Lung cancer is the leading cause of cancer-related death in the Unites States, and approximately 85% of all lung cancers are classified as non-small cell lung cancer (NSCLC), which is extremely difficult to treat and its survival rate is low. After decades of clinical trials, the most effective treatments are still those that implement the … quarry clothes

Hypoxia-induced PVT1 promotes lung cancer chemoresistance to …

WebOur results identified cisplatin resistant subpopulations of NSCLC cells with a putative stem-like signature, providing a further understanding of the cellular events associated with the cisplatin resistance phenotype in lung cancer. WebMay 11, 2012 · As shown in Figures 2a and b, apoptosis were induced in both A549 and H460 cells when they were treated with cisplatin alone (10 and 12.45%, respectively), … WebWe previously had obtained cisplatin-resistant non-small cell lung cancer (NSCLC) A549 cell lines (A549/DDP) and its parental A549 cells. 12,13 In this study, we aim to observe the difference of autophagy between A549/DDP and A549, and also determine the role autophagy plays and the underlying regulation mechanisms during DDP treatment in … quarry code of practice tasmania

GSDME enhances Cisplatin sensitivity to regress non-small cell

Icariside II enhances cisplatin-induced apoptosis by promoting ...

WebOct 1, 2024 · As the mechanism of resistance development differs depending on the cancer cell types, we aimed to evaluate the detailed mechanism of resistance to CDDP in two types of lung cancer cells: SBC-3 and A549 cells. The CDDP-resistant SBC-3/DDP and A549/DDP cells were established through continuous treatment with a gradually … WebThe A549 cells transfected with sh-PVT1 or sh-NC plasmid were seeded into the right flank of the mice, and 50 μM cisplatin was injected into the intraperitoneal every three days. Consistent with the cellular results, the tumor size, volume, and weight of the sh-PVT1 A549 cell group were significantly lower than the control group (Fig. 3F–H ). quarry collection wineWebTargeted therapy for cisplatin‐resistant lung cancer via aptamer‐guided nano‐zinc carriers containing USP14 siRNA Xinmin Zhao,1 ,2 Xianghua Wu,1 ,2 Huijie Wang,1 ,2 Songtao Lai,2 ,3 ,4 and Jialei Wang1 ,2 Xinmin Zhao 1 Department of Thoracic Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai China 2 Department of Oncology, quarry clinic kingston

"WebSep 23, 2024 · Taken together, novel toxicological-related mechanisms by AgNPs-GA were proposed involving the generation of ROS that causes DNA damage which led to programmed cell death in both A549 and BEAS-2B cells. " - Cisplatin a549

Cisplatin a549

IC 50 values for cisplatin, curcuminoid, and combination …

WebNov 29, 2024 · Cisplatin-resistant A549 non-small cell lung cancer cells can be identified by increased mitochondrial mass and are sensitive to pemetrexed treatment Yanyun Gao, … WebEffect of MiR-195-5p on the Sensitivity of A549 and A549/DDP Cells to Cisplatin Sensitivity to cisplatin in the different groups was measured as the IC 50 concentration after transfection. Growth inhibition rate of the A549 cells was 52.0% under the effect of cisplatin at an IC 50 concentration of 0.8 μg/mL but decreased to 37.6% after ...

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WebJan 12, 2024 · To evaluate whether cilastatin influences the antitumor effects of cisplatin in human cancer cells, SCID mice were injected with A549 human lung adenocarcinoma cells. The mice were treated with... WebIn addition, GA reduced the MRP2 and LRP protein expression level in A549/DDP cells. GA inhibits the proliferation, induces cell cycle arrest and apoptosis in A549/DDP cells. …

WebApr 8, 2024 · A549 cells were seeded at a density of 3×10 4 cells/well for the C group (sensitive cells) and 9×10 4 cells/well for the CIS population (resistant cells) in 96-well … WebBottom Line: Characterisation studies demonstrated a decreased proliferative capacity of lung tumour cells in response to cisplatin, increased resistance to cisplatin-induced cell death, accumulation of resistant cells in the G0/G1 phase of the cell cycle and enhanced clonogenic survival ability.The stem cell markers, Nanog, Oct-4 and SOX-2, were …

WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … WebThe addition of TAS reduced the IC 50 value of cisplatin in A549 and H1299 cells, indicating that TAS can significantly increase the sensitivity of lung cancer cells to …

WebAug 24, 2024 · We have next established xenograft model in nude mice via injecting LLC, A549, or H1299 cells, which stably overexpress or silence GSDME. Contrast to normal control cells, GSDME-overexpressed...

WebApoptosis of Cisplatin-induced A549 cells was determined by immunofluorescence and flow cytometry, respectively. Western blot was performed to detect protein levels of Prx V, Bcl … quarry coaster walesWebFeb 28, 2024 · The approximately 85% of lung cancers are non-small cell lung cancer (NSCLC), with dismal median overall survival and 5-year survival rates due to recurrence, extensive invasion and metastasis 2-4. Cisplatin (DDP) is a first-line cytotoxic agent for the treatment of human NSCLC. quarry command minecraftWebNov 19, 2013 · A549 and DU145 cells were exposed to cisplatin at an IC50 dose and ROS levels measured in Annexin V-negative subpopulation as described in Materials and Methods and in Figure S2. Antimycin A was used as … quarry clubWebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … quarry collection natural stone coastersWebThe addition of TAS reduced the IC 50 value of cisplatin in A549 and H1299 cells, indicating that TAS can significantly increase the sensitivity of lung cancer cells to cisplatin. Additionally, to determine the effects of the combination of TAS and cisplatin on the apoptosis of NSCLC cells, flow cytometry was used to detect the apoptosis rate ... quarry cockeysvilleWebJan 1, 2024 · Results: Hypoxia exacerbated the cisplatin resistance in lung cancer cells due to the increased expression of PKM2 that was observed in the exosomes secreted by hypoxic cisplatin-resistance cells. We identified that hypoxia-induced exosomal PKM2 transmitted cisplatin-resistance to sensitive NSCLC cells in vitro and in vivo. quarry cottage hermann moWebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi … quarry corchia italy