Ctll2 cell
WebInterleukin-2 (IL-2) specifically recognizes high-mannose type glycans with five or six mannosyl residues. To determine whether the carbohydrate recognition activity of IL-2 contributes to its physiological activity, the inhibitory effects of high-mannose type glycans on IL-2-dependent CTLL-2 cell proliferation were investigated. Man5GlcNAc2Asn added to … WebThe cells require IL-2 for growth and are used to assay for its presence in conditioned media. Application. CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium. RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).
Ctll2 cell
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Web目的 研究抗fas的锤头状核酶对小鼠细胞毒性T淋巴细胞(CTL)系CTLL-2细胞fas基因的表达及其介导的细胞凋亡抑制作用,探索供者淋巴细胞输注(DLI)时增强T细胞抗白血病的新途径.方法设计并合成抗fas的锤头状核酶基因,将其导入CTLL-2细胞,通过RT-PCR、Western blot和流式细胞仪分别检测核酶对CTLL-2细胞fas mRNA和Fas ... WebUsing limiting dilution analysis the HTLp frequency is determined by measuring IL-2 using the IL-2-dependent murine cell line CTLL-2. An important factor associated with the successful performance of any bioassay is the reliability of the indicator cell line used.
WebCTLL, CTLL2, CTLL(2), CT, CTL2, CTL-2, TLL 2 Mouse C57b1/6 T cell CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Mouse Blood Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells. Nature 268:154-6 PMID: 145543 … WebJul 22, 2024 · Thanks to cell proliferation assays, which use CTLL2 cells, the activities of these proteins have been validated. Human IL-2 ( 11848-H08H ) Cell proliferation assay …
WebMar 9, 2001 · Man (5)GlcNAc (2)Asn added to CTLL-2 cell cultures inhibited not only phosphorylation of tyrosine kinases but also IL-2-dependent cell proliferation. We found … WebCTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. Application CTLL-2 …
WebCTLL2 cells respond to recombinant mIL12 in the IL12-specific nature of this response. These data the growth medium indicate that the cell-based assay was comparable to the CTLL-2 cells (5 × 103) were incubated with recombi- sensitivity of the traditional splenocyte-based IFNγ nant mIL12 at different doses ranging from 0 to 12.5 ng/ release ...
http://immunservice.com/wp-content/uploads/User-Manual-WST-1-CTLL-2-WST1.CTLL150813-EN-V4.pdf grandstream product replacementWebTimothy M. Bender's 5 research works with 233 citations and 65 reads, including: Mechanism of c-Myc regulation by c-Myb in different cell lineages chinese restaurant michigan avenue chicagoWeb6.7 Cell count and Cell viability as per SOP 13214-Cell Enumeration Techniques for Mammalian Cell culture. 7.0 Assay Procedure 7.1 Perform cell count and cell viability … chinese restaurant middleton wiWebNov 1, 2009 · CTLL2 is a transformed murine cell line and its use may not reflect the activity of soluble IL-15 on lymphocytes in the blood of humans or other primate species. We therefore generated separate IL-15 dependent assays that measured the up-regulation of CD69 on the surface of CD3 − CD8 + lymphocytes in human and cynomolgus blood. grandstream pstn gatewayWebNov 4, 2024 · CTLL2 cells are dependent on IL-2 for growth and survival and are commonly used to assay IL-2 signaling events. Since CTLL2 cells require IL-2 supplemented … chinese restaurant middlesbroughWebThe CTLL-2 assay is slightly more sensitive than the ELISA, but it is not specific for IL-15, because CTLL-2 cells also respond to IL-2 and IL-4. The CTLL-2 assay can be used to measure human, simian, or murine IL-15. MeSH terms Animals Antibodies, Monoclonal / immunology Biological Assay / methods* Cell Line Cell Proliferation chinese restaurant middlebrook boltonWebT cells and the deterioration of the host immune systems are involved in ATL development (10, 26). HTLV-2 is genetically and biologically similar to HTLV-1. HTLV-2 can also immortalize primary human T cells in vitro, thereby establishing a life-long persistent infection in infected individuals, although HTLV-2 preferentially immortalizes CD8 T ... grandstream provisioning tool