Cy3 colour
WebCytiva™ Description General description Cy3 ™ Maleimide Mono-Reactive Dye 5-Pack More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Provides an alternative when CyDye ™ NHS Esters are unsuitable. WebThe Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the …
Cy3 colour
Did you know?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). See more Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. Although the name derives etymologically from terms for shades of See more Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of wavelengths which will form an image on the film, making the film See more The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the See more Cyanines have been classified in many ways: • Streptocyanines or open chain cyanines: R2N =CH[CH=CH]n-NR2 (I) • Hemicyanines: Aryl=N =CH[CH=CH]n-NR2 (II) • Closed … See more Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as See more WebAlexa Fluor® 555 vs. Cy®3 dye Alexa Fluor® 555 dye has higher photostability and quantum yield than tetramethylrhodamine isothiocyanate (TRITC) and rhodamine derivatives, which allow more time for observation and image capture. Alexa Fluor® 555 dye also has superior fluorescence output per protein or nucleic acid conjugate, surpassing …
WebThe color of Cy3 is pinkish red and that of Cy5 is blue to the naked eye. However emission spectrum of Cy3 is in the greenish yellow and that of Cy5 is in the Red region. WebCy3 Dye If the Cy3 dye is sufficiently close (2–9nm) to the Cy5 (acceptor) dye, energy transfer can occur resulting in a mixture of emitted light coming from the donor and …
WebInvitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ... WebJul 1, 2024 · Answer. Cy3 is orange in color. It fluoresces greenish yellow at 555 nm excitation and 570 nm emission. Cy3 belongs to the Cyanine family of synthetic …
WebCyanine Dye 3. Usually, cyanine 3 (Cy3) and cyanine 5 (Cy5) with excitation and emission maxima wavelengths of 555/569 and 651/670 nm are respectively used to label nucleotides (usually dCTP or dUTP) and incorporated them into DNA of interest using DNA polymerase.
WebThe cyanine dye Cy3 is a popular fluorophore used to probe the binding of proteins to nucleic acids as well as their conformational transitions. Nucleic acids labeled only with Cy3 can often be ... cell has half the chromosomes of aWebFluorescent dyes. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. … buy cars through usaaWebCy3 carboxylic acid, fluorescent dye, Cy3 C32H40N2O5S CID 131953006 - structure, chemical names, physical and chemical properties, classification, patents, literature, … cellhawk loginhttp://www.stargraydye.com/portal/news/info/id/442.html buy cars through consumer reportsWebApr 13, 2024 · The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has … buy cars to build creditWebApr 4, 2024 · 产品名称：菁染料CY3标记透明质酸 英文名称：Cy3-Hylauronic 外观状态：红色粉末 激发发射波长：550/580nm左右 规格：mg 保存：-20℃ 分子量：10K、20K、30K等（不同分子量可定制） CY3-透明质酸荧光染料是一种常用的生物荧光染料，CY3-透明质酸结合了透明质酸的保湿作用和CY3的荧光标记功能，可用于研究细胞外基质的组成和结 … cell has a true membrane-enclosed nucleusWebOct 9, 2024 · To examine their mitochondria targeting ability, HeLa cells were incubated with 10 μM Cy3, Cy5 or Cy3–Cy5 (Fig. 1a and b) followed by live-cell confocal imaging. Cy3–Cy5 was chosen to investigate if having two fluorophores would enhance uptake and targeting effect. This is of interest for designing Cy3–Cy5-based FRET pairs as … cellhashr